Comparison List


a.k.a. Dronpa-V60A, fast-forming Dronpa

ffDronpa is a photoswitchable green fluorescent protein published in 2014, derived from Echinophyllia sp. SC22. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Echinophyllia sp. SC22 25.5 kDa -

FPbase ID: 9U6KU


State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
On 503 517 105,000 0.75 78.75 5.0 15.0  


From To Switch λ
Off On 405
On Off 488


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ffDronpa Sequence

ffDronpa was derived from Dronpa with the following mutations: V60A



By observing the brightness of bacterial colonies expressing randomly mutated Dronpa genes, we identified Dronpa-V60A, a monomeric Dronpa mutant with increased in-colony brightness. In vitro maturation measurements showed that Dronpa-V60A forms almost three times faster than Dronpa, with a maturation half-time of less than 15 min. We thus called this mutant “fast-forming” or ffDronpa.

Moeyaert et al. (2014)

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