a.k.a. Dronpa-V60A, fast-forming Dronpa
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|Tetramer||Echinophyllia sp. SC22||25.5 kDa||-|
|State||Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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ffDronpa was derived from Dronpa with the following mutations: V60A
By observing the brightness of bacterial colonies expressing randomly mutated Dronpa genes, we identified Dronpa-V60A, a monomeric Dronpa mutant with increased in-colony brightness. In vitro maturation measurements showed that Dronpa-V60A forms almost three times faster than Dronpa, with a maturation half-time of less than 15 min. We thus called this mutant “fast-forming” or ffDronpa.