a.k.a. Dronpa-V60A, fast-forming Dronpa
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| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Echinophyllia sp. SC22 | 25.5 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| Off | ||||||||
| On | 503 | 517 | 105,000 | 0.75 | 78.75 | 5.0 | 15.0 |
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ffDronpa was derived from Dronpa with the following mutations: V60A
By observing the brightness of bacterial colonies expressing randomly mutated Dronpa genes, we identified Dronpa-V60A, a monomeric Dronpa mutant with increased in-colony brightness. In vitro maturation measurements showed that Dronpa-V60A forms almost three times faster than Dronpa, with a maturation half-time of less than 15 min. We thus called this mutant “fast-forming” or ffDronpa.
Moeyaert et al. (2014)
(2014). ACS Nano, 8(2) , 1664-1673. doi: 10.1021/nn4060144. Article Pubmed
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