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Comparison List

Bluebonnet2

Bluebonnet2 is a basic (constitutively fluorescent) blue fluorescent protein published in 2020, derived from Entacmaea quadricolor.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.5 kDa -

FPbase ID: JSPPN

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
399 454          
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Photostability

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Bluebonnet2 Sequence

BLAST

Bluebonnet2 was derived from secBFP2 with the following mutations: T18W/A26C/S28A/S114V/V124W/T127P/N158T/N173T
amino acid numbers relative to eqFP578. show relative to secBFP2

MSEELIKENMHMKLYMEGWVDNHHFKCTAEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGVLIYNVKIRGWDFPSNGPVMQKKTLGWEAFTETLYPADGGLEGRTDMALKLVGGSHLIATAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANDETYVEQHEVAVARYSDLPSKLGHKLN

Excerpts

No excerpts have been added for Bluebonnet2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Discovery of Novel Gain-of-Function Mutations Guided by Structure-Based Deep Learning

Shroff R, Cole Aw, Diaz Dj, Morrow Br, Donnell I, Annapareddy A, Gollihar J, Ellington Ad, Thyer R

(2020). ACS Synthetic Biology, 9(11) , 2927-2935. doi: 10.1021/acssynbio.0c00345. Article   Pubmed

Additional References

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External Resources

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