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Comparison List

mCerulean2.N

mCerulean2.N is a basic (constitutively fluorescent) cyan fluorescent protein published in 2011, derived from Aequorea victoria. It has low acid sensitivity.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.7 kDa -

FPbase ID: WQ6TU

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
440 484 49,000 0.48 23.52 4.5   2.63
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Photostability

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mCerulean2.N Sequence

BLAST

mCerulean2.N was derived from mCerulean2 with the following mutations: T203I
amino acid numbers relative to avGFP. show relative to mCerulean2

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

Excerpts

No excerpts have been added for mCerulean2.N
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

Markwardt Ml, Kremers G-J, Kraft Ca, Ray K, Cranfill Pjc, Wilson Ka, Day Rn, Wachter Rm, Davidson Mw, Rizzo Ma

(2011). PLoS ONE, 6(3) , e17896. doi: 10.1371/journal.pone.0017896. Article   Pubmed

Additional References

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External Resources

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