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Comparison List

mCyRFP1

similar: CyRFP1

mCyRFP1 is a basic (constitutively fluorescent) yellow fluorescent protein published in 2016, derived from Entacmaea quadricolor. It has moderate acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.4 kDa -

FPbase ID: UQH8C

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
528 594 27,000 0.65 17.55 5.6   3.71
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
45.0   Laviv et al. (2016)
A caution on interpretation of photostability measurements
Add photostability info

mCyRFP1 Sequence

BLAST

mCyRFP1 was derived from CyRFP1 with the following mutations: N41A/A161K
amino acid numbers relative to eqFP578. show relative to CyRFP1

MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTARIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGGVLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCDKKLKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARYSNLGGGMDELYK
GenBank: AOY07765

Excerpts

No excerpts have been added for mCyRFP1
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins

Laviv T, Kim Bb, Chu J, Lam Aj, Lin Mz, Yasuda R

(2016). Nature Methods, 13(12) , 989-992. doi: 10.1038/nmeth.4046. Article   Pubmed

Additional References

  1. A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Kim Bb, Wu H, Hao Ya, Pan M, Chavarha M, Zhao Y, Westberg M, St-Pierre F, Wu Jc, Lin Mz

    (2022). Scientific Reports, 12(1) , . doi: 10.1038/s41598-022-07313-1. Article

External Resources

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