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Comparison List

mRhubarb719

mRhubarb719 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2019, derived from Rhodopseudomonas palustris. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.5 kDa Biliverdin

FPbase ID: TUP2T

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
700 719 83,768 0.068 5.7      
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Photostability

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mRhubarb719 Sequence

BLAST
MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRHTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHSPASYIPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACELVAQVLARAIGVMEE

Excerpts

No excerpts have been added for mRhubarb719
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

mRhubarb: Engineering of monomeric, red-shifted, and brighter variants of iRFP using structure-guided multi-site mutagenesis

Rogers Oc, Johnson Dm, Firnberg E

(2019). Scientific Reports, 9(1) , 15653. doi: 10.1038/s41598-019-52123-7. Article   Pubmed

Additional References

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External Resources

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