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Comparison List

shBFP

shBFP is a basic (constitutively fluorescent) blue fluorescent protein published in 2016, derived from Stichodactyla haddoni.
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shBFP Spectrum Fluorescent protein shBFP excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Weak dimer Stichodactyla haddoni 25.5 kDa -

FPbase ID: QGZZT

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
401 458 15,407 0.79 12.17      
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Photostability

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shBFP Sequence

BLAST

shBFP was derived from shCP with the following mutations: E63L/Y64L

MAGLLKESMRIKMDMEGTVNGHYFKCEGEGDGNPFTGTQSMRIHVTEGAPLPFAFDILAPCCLLGSRTFIHHTAGIPDFFKQSFPEGFTWERTTTYEDGGILTAHQDTSLEGNCLIYKVKVLGTNFPADGPVMKNKSEGWEPCTEVVYPDNGVLCGRNVMALKVGDRRLICHLYSSYKSKKAIRALTMPGFHFTDIRLQMPRKKKDEYFELYEASVARYSDLPEKAN

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for shBFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Blue fluorescent protein derived from the mutated purple chromoprotein isolated from the sea anemone Stichodactyla haddoni

Chiang C-Y, Lin C-Y, Chen Y-T, Tsai H-J

(2016). Protein Engineering Design and Selection, 29(11) , 523-530. doi: 10.1093/protein/gzw041. Article   Pubmed

Additional References

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External Resources

Change history

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