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Comparison List

mRFP1-Q66C

a.k.a. mRFP1_Orange

mRFP1-Q66C is a basic (constitutively fluorescent) red fluorescent protein published in 2006, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 25.4 kDa -

FPbase ID: MSSUL

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
559 580 31,800 0.33 10.49   10.8  
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Photostability

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mRFP1-Q66C Sequence

BLAST

mRFP1-Q66C was derived from mRFP1 with the following mutations: Q66C

MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFCYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA

Excerpts

No excerpts have been added for mRFP1-Q66C
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

An improved mRFP1 adds red to bimolecular fluorescence complementation

Jach G, Pesch M, Richter K, Frings S, Uhrig Jf

(2006). Nature Methods, 3(8) , 597-600. doi: 10.1038/nmeth901. Article   Pubmed

Additional References

  1. Overcoming chromoprotein limitations by engineering a red fluorescent protein

    Bao L, Menon Pnk, Liljeruhm J, Forster Ac

    (2020). Analytical Biochemistry, 611, 113936. doi: 10.1016/j.ab.2020.113936. Article   Pubmed

External Resources

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