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Comparison List

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Lissamine

a.k.a. dimeric vsfGFP, vGFP-0

This protein has unmoderated changes. Click here to view the last moderated version of this protein from Dec 8, 2018 17:53:39. Or view the full change history

Lissamine is a basic (constitutively fluorescent) red fluorescent protein published in 2015, derived from Aequorea victoria. It has low acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Dimer	Aequorea victoria	38.4 kDa	-

FPbase ID: BH9MB

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
575	593	209,916	0.76	159.54	4.84

Edit States/Attributes

Photostability

No photostability measurements available ... add one!

Lissamine Sequence

BLAST

Lissamine was derived from Superfolder GFP with the following mutations: T230_K238delinsAQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVST230_K238delinsAQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVS

MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGIAQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVS

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for Lissamine
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Rational Structure-Based Design of Bright GFP-Based Complexes with Tunable Dimerization

Eshaghi M, Sun G, Grüter A, Lim Cl, Chee Yc, Jung G, Jauch R, Wohland T, Chen Sl

(2015). Angewandte Chemie International Edition, 54(47) , 13952-13956. doi: 10.1002/anie.201506686. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for Lissamine
Search RIKEN BRC for Lissamine

Change history

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