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Comparison List

mRFP1_Yellow

mRFP1_Yellow is a basic (constitutively fluorescent) fluorescent protein published in 2020, derived from Discosoma sp..

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Oligomerization Organism Molecular Weight Cofactor
? Discosoma sp. 25.4 kDa -

FPbase ID: 8O716

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
498            
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Photostability

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mRFP1_Yellow Sequence

BLAST

mRFP1_Yellow was derived from mRFP1 with the following mutations: F65I/Q66L

MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQILYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA

Excerpts

mRFP1_Yellow … had emissions <5% of mRFP1 and thus [was] considered nonfluorescent.

Bao et al. (2020)

Primary Reference

Overcoming chromoprotein limitations by engineering a red fluorescent protein

Bao L, Menon Pnk, Liljeruhm J, Forster Ac

(2020). Analytical Biochemistry, 611, 113936. doi: 10.1016/j.ab.2020.113936. Article   Pubmed

Additional References

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External Resources

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