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WiPhy2

WiPhy2 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2015, derived from Deinococcus radiodurans. It requires the cofactor biliverdin for fluorescence.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Deinococcus radiodurans 37.1 kDa Biliverdin

FPbase ID: R4JFX

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
696 719 0.087       0.78
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Photostability

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WiPhy2 Sequence

BLAST

WiPhy2 was derived from DrCBD with the following mutations: F145S/D207L/Y263F/L311E/L314E

MASMTGGQQMGRGSMSRDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADGHSGEVLQMSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLDWPAAGHLSLTVHRVGELLILEFEPTEAWDSTGPHALRNAMSALESAPNLRALAEVATQTVRELTGFDRVMLYKFAPDATGEVIAEARREGLHAFLGHRFPASLIPAQARALYTRHLLRLTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQFLRNMGVGSSLSVSVVVGGQLWGLIACHHQTPYVLPPDLRTTLEYLGRELSEQVQVKEALEHHHHHH

Structure

Deposited: ,
Chromophore:

Excerpts

"This new phytofluor maintains its fluorescent properties over a broad pH range and does not suffer from photobleaching. WiPhy2 achieves the best compromise to date between high fluorescence quantum yield and long illumination wavelength in this class of fluorescent proteins."

Lehtivuori et al. (2015)

Primary Reference

Removal of Chromophore-Proximal Polar Atoms Decreases Water Content and Increases Fluorescence in a Near Infrared Phytofluor

Lehtivuori H, Bhattacharya S, Angenent-Mari Nm, Satyshur Ka, Forest Kt

(2015). Frontiers in Molecular Biosciences, 2, 65. doi: 10.3389/fmolb.2015.00065. Article   Pubmed

Additional References

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