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Comparison List

Wi-Phy

a.k.a. Wisconsin infrared phytofluor

Wi-Phy is a basic (constitutively fluorescent) near ir fluorescent protein published in 2011, derived from Deinococcus radiodurans. It requires the cofactor biliverdin for fluorescence.

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Aggregation Organism Molecular Weight Cofactor
Monomer Deinococcus radiodurans 36.1 kDa Biliverdin

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
701 719 92,991 0.047 4.37      

Photostability

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Wi-Phy Sequence

MASMTGGQQMGRGSMSRDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADGHSGEVLQMSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLDWPAAGHLSLTVHRVGELLILEFEPTEAWDSTGPHALRNAMSALESAPNLRALAEVATQTVRELTGFDRVMLYKFAPDATGEVIAEARREGLHAFLGHRFPASHIPAQARALYTRHLLRLTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQFLRNMGVGSSLSVSVVVGGQLWGLIACHHQTPYVLPPDLRTTLEYLGRELSEQVQVKEA

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for Wi-Phy
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Structure-guided Engineering Enhances a Phytochrome-based Infrared Fluorescent Protein

Auldridge Me, Satyshur Ka, Anstrom Dm, Forest Kt

(2011). Journal of Biological Chemistry, 287(10) , 7000-7009. doi: 10.1074/jbc.m111.295121. Article

Additional References

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External Resources

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