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Comparison List

sg12

sg12 is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 1997, derived from Aequorea victoria.

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Aggregation Organism Molecular Weight Cofactor
? Aequorea victoria 27.0 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
396 508 18,300 0.85 15.55      

Photostability

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sg12 Sequence

sg12 was derived from Q80R with the following mutations: M1_S2insA/F64L

MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Structure

Deposited: ,
Chromophore (SYG):

Excerpts

No excerpts have been added for sg12
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

The structural basis for spectral variations in green fluorescent protein

Palm Gj, Zdanov A, Gaitanaris Ga, Stauber R, Pavlakis Gn, Wlodawer A

(1997). Nature Structural & Molecular Biology, 4(5) , 361-365. doi: 10.1038/nsb0597-361. Article

Additional References

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External Resources

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