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Comparison List

pHuji

pHuji is a basic (constitutively fluorescent) red fluorescent protein published in 2014, derived from Discosoma sp.. It has high acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 27.0 kDa -

FPbase ID: SS15T

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
566 598 31,000 0.22 6.82 7.7    

Photostability

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pHuji Sequence

pHuji was derived from mApple with the following mutations: K158Y
amino acid numbers relative to DsRed. show relative to mApple

MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALYSEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAEGRHSTGGMDELYK

Excerpts

No excerpts have been added for pHuji
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis

Shen Y, Rosendale M, Campbell Re, Perrais D

(2014). The Journal of Cell Biology, 207(3) , 419-432. doi: 10.1083/jcb.201404107. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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