No spectrum has been submitted ... add a spectrum!
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| ? | Aequorea victoria | 26.9 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| acidic | 475 | 509 | ||||||
| alkaline | 395 | 509 |
No photostability measurements available ... add one!
pHluorin2 was derived from pHluorin, ratiometric with the following mutations: F64L
Using GFP2 as a template, an enhanced ratiometric pHluorin (pHluorin2) construct was developed to contain fully mammalianized codons, the F64L mutation and ten of the thirteen pHluorin-specific mutations. As a result, pHluorin2 displays markedly higher flores-cence when compared to pHluorin while maintaining the ratiometric pH-sensitivity.
Mahon (2011)
Analogous to the development of the superecliptic pHluorin, inclusion of mammalianized codons and the F64L mutation in the ratiometric pHluorin backbone markedly enhances florescence while maintaining the desired pH-sensitivity, yielding a super-ratiometric variant called pHluorin2.
Mahon (2011)
(2011). Advances in Bioscience and Biotechnology, 02(03) , 132-137. doi: 10.4236/abb.2011.23021. Article Pubmed
(2022). ACS Chemical Biology, 17(1) , 240-251. doi: 10.1021/acschembio.1c00907. Article Pubmed
Something missing or incorrect? Submit a change Submit a change