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mGold2t

mGold2t is a basic (constitutively fluorescent) yellow fluorescent protein published in 2025, derived from Aequorea victoria. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity.
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mGold2t Spectrum Fluorescent protein mGold2t excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.9 kDa -

FPbase ID: HUE6V

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
515 527 126,000 0.7 88.2 5.4 13.1 2.95
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Photostability

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mGold2t Sequence

BLAST

mGold2t was derived from mGold2s with the following mutations: S205T
amino acid numbers relative to avGFP. show relative to mGold2s

MASKGEELFTGVVPILVELDGDINGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTSLGYGLQCFARYPDNMKRHDFFKSAMPEGYVQERTIFFEDDGNYKTRAEVKFEGGTLVNRVELKGIDFKEDGNILGHKLEYNYNCHNVFITADRQQNGIKVNFKIRHNIEVGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQTKLSKDPNEKRDHMVLLEFVTAAGITLSMDELYK
GenBank: PP826966

Excerpts

No excerpts have been added for mGold2t
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Bright and photostable yellow fluorescent proteins for extended imaging

Lee J, Lai S, Yang S, Zhao S, Blanco Fa, Lyons Ac, Merino-Urteaga R, Ahrens Jf, Nguyen Na, Liu H, Liu Z, Lambert Gg, Shaner Nc, Chen L, Tolias Kf, Zhang J, Ha T, St-Pierre F

(2025). Nature Communications, 16(1) , 3241. doi: 10.1038/s41467-025-58223-5. Article   Pubmed

Additional References

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External Resources

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