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DsRed.M1

This protein has unmoderated changes.

DsRed.M1 is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Discosoma sp..

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Discosoma sp.	25.4 kDa	-

FPbase ID: N33JN

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
558	587	27,300	0.14	3.82			1.4

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Photostability

No photostability measurements available ... add one!

DsRed.M1 Sequence

BLAST

DsRed.M1 was derived from DsRed.T4 with the following mutations: A2D/S3N/S4T/R13Q/E26Y/R36K/K47Q/V71A/K83M/K92T/V96S/T106E/T108Q/C117T/I125K/S131A/M141A/R149K/R153Q/H162S/K163H/L174T/V175C/E176D/S179T/I180V/M182K/Y192N/Y193H/S203N/I210V/R216H/G219A/H222S/L223G/F224S/L225Q A2D/S3N/S4T/R13Q/E26Y/R36K/K47Q/V71A/K83M/K92T/V96S/T106E/T108Q/C117T/I125K/S131A/M141A/R149K/R153Q/H162S/K163H/L174T/V175C/E176D/S179T/I180V/M182K/Y192N/Y193H/S203N/I210V/R216H/G219A/H222S/L223G/F224S/L225Q

MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAKLQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGVVEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISHALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHSGSQ

Structure

PDB ID

Deposited: ,
Chromophore (QYG):

Excerpts

No excerpts have been added for DsRed.M1
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants

Strongin De, Bevis B, Khuong N, Downing Me, Strack Rl, Sundaram K, Glick Bs, Keenan Rj

(2007). Protein Engineering, Design and Selection, 20(11) , 525-534. doi: 10.1093/protein/gzm046. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for DsRed.M1
Search RIKEN BRC for DsRed.M1

Change history

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