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Comparison List

BP02

BP02 is a basic (constitutively fluorescent) uv fluorescent protein published in 2020, derived from synthetic construct.

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Oligomerization Organism Molecular Weight Cofactor
Monomer synthetic construct 3.5 kDa -

FPbase ID: 7XHKJ

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
342 382 4,494 0.1139 0.51     0.69
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Photostability

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BP02 Sequence

BLAST
ELSKETALKKSFKFLVLIILWNNTVDAIHI

Excerpts

Full sequence: GLU-LEU-SER-LYS-GLU-THR-ALA-LEU-LYS-LYS-SER-PHE-LYS-PHE-LEU-VAL-LEU-ILE-ILE-LEU-AZA-ASN-ASN-THR-VAL-ASP-ALA-ILE-HIS-ILE. In the prescribed field 21st residue is given as W. It is actually Azulenyl alanine (AZA, Z). ELsKETaLKKsFkFlVlIiLZnNtvDaIhI. Lower case in the sequence indicates D-amino acids.

Prakash et al. (2020)

Azulenyl alanine (AZA, Z) is an unnatural amino acid.

Prakash et al. (2020)

Primary Reference

De Novo Designed Heterochiral Blue Fluorescent Protein

Prakash V, Ranbhor R, Ramakrishnan V

(2020). ACS Omega, 5(41) , 26382-26388. doi: 10.1021/acsomega.0c02574. Article   Pubmed

Additional References

  1. Painting proteins blue: β-(1-azulenyl)-l-alanine as a probe for studying protein–protein interactions

    Moroz Ys, Binder W, Nygren P, Caputo Ga, Korendovych Iv

    (2013). Chem. Commun., 49(5) , 490-492. doi: 10.1039/c2cc37550h. Article   Pubmed

External Resources

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