Microscopes on FPbase are collections of
optical
configurations, including filters, light sources, lasers, and
detectors, meant to represent the hardware of a specific microscope or other imaging device. Once you save your
microscope configuration, you get a personalized spectra-viewer app that you can use to visualize the efficiency of
excitation, collection, and predicted brightness of any of the fluorophores in FPbase for your hardware. (As a bonus,
you can also quickly recall the filter settings from any of your optical configs in the general FPbase spectra viewer) You can
create as many different microscope pages as you'd like and you can share the link to your microscope page with
anyone, or embed the app in your website for others to use.
For more information on building a microscope page, see the
documentation.
Fluorophore Efficiency Reports
Once you have saved the components of your microscope, you can generate a "fluorophore efficiency report": a
prediction for excitation and collection efficiency for every fluorophore in the database for which spectral data is
available, matched with every optical configuration in your scope. Either click the icon next to the corresponding microscope here, or find the report button at
the bottom of any microscope page.
Example microscopes:
Example Yokogawa SetupAn example Yokogawa spinning disc setup with inverted dichroics (that reflect emission)
Example Widefield (Sedat)An example Sedat configuration with single-band exciters/emitters and a couple multiband dichroics
HIC GE INCELLThe GE IN CELL Analyzer 2200 is a super-fast and sensitive widefield cell imaging system fine-tuned to the needs of high content screening –
Apr 24, 2025
HIC Leica StereoscopeThe Leica MZ16FA is a mostly automated planapochromatic fluorescence stereo microscope. The filter changer, zoom, fluorescence shutter and double iris aperture are computer controlled which allows multifluorescence experiments to be repeated quicly and accurately. Focus is manual. –
Apr 24, 2025
HIC Zeiss 800With 4 lasers (405nm, 488nm, 561nm 633nm) and 3 GaAsP detectors this upright scope will be useful for cleared tissue, zebrafish, and general fixed specimens. The emission path uses variable dichroic mirrors to achieve flexibility of bandpass adjustment similar to spectral detectors that use a prism or a diffraction grating. The upright configuration of the scope allows the use of wet preparations imaged with dipping objectives. –
Apr 24, 2025
HIC Fluoview1000The Olympus FluoView FV1000 is designed for high-resolution, confocal observation of both fixed and living cells. The Fluoview is mounted on an inverted IX-81 microsocpe. –
Apr 24, 2025
HIC Zeiss 880The Zeiss 880 confocal laser scanning microscope offers high sensitivity, enhanced resolution in x, y and z and high image-acquisition speed. –
Apr 24, 2025
HIC Keyence BZ-X810Simple-to-use inverted widefield microscope for brightfield and fluorescence imaging of fixed and live samples. –
Apr 24, 2025
HIC Olympus IX-81The Olympus IX-81 is an inverted widefield microscope equipped with a motorized stage, filter wheels and shutter for fast and accurate multifluorescence image acquisitions. –
Apr 24, 2025
HIC VS200 SlideScannerAutomated slide scanner for up to six 1x3in or three 2x4in slides at a time. Great for tissue sections and imaging very large numbers of tissue culture cells for robust statistics. –
Apr 24, 2025