With 4 lasers (405nm, 488nm, 561nm 633nm) and 3 GaAsP detectors this upright scope will be useful for cleared tissue, zebrafish, and general fixed specimens. The emission path uses variable dichroic mirrors to achieve flexibility of bandpass adjustment similar to spectral detectors that use a prism or a diffraction grating. The upright configuration of the scope allows the use of wet preparations imaged with dipping objectives.