| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Aequorea victoria | 26.9 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 433 | 474 | 30,000 | 0.56 | 16.8 | 4.5 | 2.6 |
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We created monomeric optimized variants of ECFP and EYFP, which fold faster and more efficiently at 37°C and have superior solubility and brightness. Bacteria expressing SCFP3A were 9-fold brighter than those expressing ECFP and 1.2-fold brighter than bacteria expressing Cerulean. In HeLa cells, the improvements were less pronounced; nonetheless, cells expressing SCFP3A and SYFP2 were both 1.5-fold brighter than cells expressing ECFP and EYFP(Q69K), respectively.
Kremers et al. (2006)
(2006). Biochemistry, 45(21) , 6570-6580. doi: 10.1021/bi0516273. Article Pubmed
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