| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Dimer | Aequorea australis | 25.7 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 504 | 510 | 170,000 | 0.97 | 164.9 | 6.0 |
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We were surprised to discover a second green-emitting FP in A. cf. australis, AausFP1, that shares only 53% amino acid identity with avGFP (see Fig. 4). AausFP1 is the brightest FP discovered to date, with a nearly perfect quantum yield (0.97) and a peak extinction coefficient of 170,000 M−1cm−1, making it nearly fivefold brighter than EGFP on a per-molecule basis. These already extraordinary properties are further bolstered by a low fluorescence pKa (4.4) and unusually narrow excitation and emission peaks (see Fig. 3; the emission peak of AausFP1 has a full width at half maximum (FWHM) of 19nm, compared to 32nm for EGFP).
Lambert et al. (2019)
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