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Comparison List

miRFP670-2

a.k.a. monomeric iRFP670-2

miRFP670-2 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris CGA009. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris CGA009 34.5 kDa Biliverdin

FPbase ID: 1KUE6

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
643 670 103,000 0.136 14.01 4.5   1.42
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
310.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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miRFP670-2 Sequence

BLAST

miRFP670-2 was derived from iRFP670 with the following mutations: E294K/M295R/D298E/F299R/L302T

MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGAFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHRHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVMLYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRIVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEPRAVPMAQRVAAKRFAERLSTHFTAAHHQR

Excerpts

No excerpts have been added for miRFP670-2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Matlashov Me, Shcherbakova Dm, Alvelid J, Baloban M, Pennacchietti F, Shemetov Aa, Testa I, Verkhusha Vv

(2020). Nature Communications, 11(1) , 239. doi: 10.1038/s41467-019-13897-6. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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