2F 현미경

FPbase

위에 필터용 레이져 = Violet, Cyan, Green, Red // 아래 필터용 레이져 = Blue, Teal, Yellow, nlR

click and drag to zoom

linear log

Fluorophore

2p

Brightness:

Configurations

Light Source

Detector

Excitation Filters

Leica P DAPI/FITC/TRITC/Cy5 Ex 391/32-479/33-554/24-638/31 (11525418)

Leica P CFP/YFP/mCherry/Cy7 Ex 436/28-506/21-578/24-730/40 (11525416)

Dichroic Mirrors invert

Leica P DAPI/FITC/TRITC/Cy5 Di 415-500-572-660 (11525418)

Leica P CFP/YFP/mCherry/Cy7 Di 459-523-598-763 (11525416)

Emission Filters

Leica P DAPI/FITC/TRITC/Cy5 Em 435/30-519/25-594/32-695/58 (11525418)

Leica P CFP/YFP/mCherry/Cy7 Em 473/22-539/24-641/78-810/80 (11525416)

Fluorophore Efficiency Report

Settings

Note: When scaling to EC or QY, dyes/probes lacking EC/QY values in the database will disappear on the graph!

This link will reconstruct your current configuration:

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