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Comparison List

mRFP1_Violet

mRFP1_Violet is a basic (constitutively fluorescent) red fluorescent protein published in 2020, derived from Discosoma sp..

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Oligomerization Organism Molecular Weight Cofactor
? Discosoma sp. 25.4 kDa -

FPbase ID: HBEF2

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
586 619          

Photostability

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mRFP1_Violet Sequence

mRFP1_Violet was derived from mRFP1 with the following mutations: F65C/Q66M

MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQCMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA

Excerpts

No excerpts have been added for mRFP1_Violet
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Overcoming chromoprotein limitations by engineering a red fluorescent protein

Bao L, Menon Pnk, Liljeruhm J, Forster Ac

(2020). Analytical Biochemistry, 611, 113936. doi: 10.1016/j.ab.2020.113936. Article   Pubmed

Additional References

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External Resources

Change history

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